Coding

Part:BBa_K2557000:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-04)


Anti-GFP-mnotch-TEV protease-NLS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 409
    Illegal PstI site found at 453
    Illegal PstI site found at 772
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 16
    Illegal PstI site found at 409
    Illegal PstI site found at 453
    Illegal PstI site found at 772
    Illegal NotI site found at 1570
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 409
    Illegal PstI site found at 453
    Illegal PstI site found at 772
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 409
    Illegal PstI site found at 453
    Illegal PstI site found at 772
    Illegal NgoMIV site found at 208
    Illegal NgoMIV site found at 913
    Illegal NgoMIV site found at 1090
    Illegal NgoMIV site found at 1628
    Illegal AgeI site found at 25
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1249


Design Notes

Since we need TEV protease to enter the nucleus, we added SV40 NLS to the back of the TEV protease.


Source

We borrowed the Anti-GFP-mnotch-tetR-VP64-V2 plasmid from Fudan University. After the TEV protease sequence used by the iGEM17_Oxford group was found on the iGEM website, it was sent to Kingsray for synthesis.

References